Polyclonal Anti- Cyclin D1 antibody

Catalog# YPA2128

Lot # Check on the product label

Size 100 μl

Isotype IgG

Host Rabbit

Reactivity 

Human, mouse, rat

Immunogen A synthesized peptide derived from human Cyclin D1, corresponding to a region within C-terminal amino acids.

Purification Purified by peptide affinity chromatography

Content Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Recommend Application

Western Blot, WB (1:250-1:1000)

Immunohistochemistry, IHC (1:20-1:100)

Immunocytochemistry, ICC/IF (1:50-250)

Other applications have not been tested.

The optimal dilutions should be determined by end user.

Storage Instruction 

Ship at 2-8°C, when receipt, aliquot and store at -20°C for one year.

Avoid repeated freeze and thaw cycles.

Background

cyclin-D1 is a protein that in humans is encoded by the CCND1 gene. Cyclin D1 belongs to the core cell cycle machinery, and it is frequently overexpressed in human cancers. Cyclin D1 encodes the regulatory subunit of a holoenzyme that phosphorylates and inactivates the retinoblastoma protein and promotes progression through the G1-S phase of the cell cycle. CCND1 associates with and regulates transcription factors, coactivators, and corepressors that govern histone acetylation and chromatin remodeling proteins. CCND1 also has roles in cellular growth, metabolism, and cellular differentiation. Amplification or overexpression of cyclin D1 plays pivotal roles in the development of a subset of human cancers including parathyroid adenoma, breast cancer, colon cancer, lymphoma, melanoma, and prostate cancer.                           

Reference

1. Bienvenu, F., Jirawatnotai, S., Elias, J. E., Meyer, C. A., Mizeracka, K., Marson, A., Frampton, G. M., Cole, M. F., Odom, D. T., Odajima, J., Geng, Y., Zagozdzon, A., Jecrois, M., Young, R. A., Liu, X. S., Cepko, C. L., Gygi, S. P., Sicinski, P. Transcriptional role of cyclin D1 in development revealed by a genetic-proteomic screen. Nature 463: 374-378, 2010.

2. Fu, M., Wang, C., Li, Z., Sakamaki, T., Pestell, R. G. Minireview: Cyclin D1: normal and abnormal functions. Endocrinology 145: 5439-5447, 2004.