Monoclonal Anti-MBP Tag Antibody

Catalog# BTL1014

Lot # Check on the product label

Size 100μg

Isotype IgG1

Host Mouse

Reactivity All

Product Form Liquid (1mg/ml)

Immunogen 

Recombinant MBP protein with full length.

Recommend Application

Western Blot (1:1000-10000)

Other applications have not been tested.

The optimal dilutions should be determined by end user.

Storage Buffer

In PBS (pH7.4), 50% Glycerol and 0.02% Sodium Azide.

Storage Instruction 

Store at 2-8°C for 1 month. Or aliquot and store at -20°C or below for long term.

Avoid repeated freeze and thaw cycles.

Background

Maltose-Binding Protein (MBP) is a part of the maltose/maltodextrin system of Escherichia coli, which is responsible for the uptake and efficient catabolism of maltodextrins. It has an approximate molecular mass of 42.5 kilodaltons. MBP is used to increase the solubility of recombinant proteins expressed in E. coli. In these systems, the protein of interest is often expressed as a MBP-fusion protein, preventing aggregation of the protein of interest. In addition, MBP can itself be used as an affinity tag for purification of recombinant proteins. The fusion protein binds to amylose columns while all other proteins flow through. The MBP-protein fusion can be purified by eluting the column with maltose.

Reference

1. Claude V. Maina, Paul D. Riggs, Andres G. Grandea III, Barton E. Slatko, Laurie S. Moran, John A. Tagliamonte, Larry A. McReynolds, di Guan Chu. "An Escherichia coli vector to express and purify foreign proteins by fusion to and separation from maltose-binding protein" Gene Volume 74, Issue 2, 30 December 1988, Pages 365–373