2×Taq PCR MasterMix (with dye)

Catalog# BPC1036-1

Lot # Check on the product label

Size: 1 ml

Concentration: 0.1 U/µl

Storage and Expiration: Store at -20°C.

Application: For Genetic testing, semi-quantitative PCR experiments and Trace DNA testing.

Description: 2x Taq MasterMix is a premixed 2x concentrated solution of Taq DNA Polymerase, dNTPs, reaction buffer, MgCl₂ and stabilizers. 2x Taq Master Mix contains all components for PCR, except DNA template and primers. It is ideally suited to routine PCR applications from templates including pure DNA solutions, bacterial colonies, and cDNA products. It can amplify up to 4 kb from genomic DNA or up to 5 kb from lambda DNA. For a 50 μl reaction, simply add 25 μl of 2x Taq MasterMix to primers, DNAtemplate and PCR-Qualified H₂O.

Quality Control: No activity of exogenous nuclease is detected; Detection of host-free residual DNA by PCR method; Single-copy gene in human genome could be amplified effectively; No significant activity change when stored at room temperature for one week.

Content: Taq DNA Polymerase (recombinant): 0.05 units/µl; MgCl 2: 4mM; dNTPs (dATP, dCTP, dGTP, dTTP): 0.4mM.

Examples of reaction

(Note: The following example for reference only. Set the optimal reaction conditions according to the actual situation.)

1. Use genomic DNA as template to amplify 1kb fragment, the reaction system is 25µl. (If the reaction system is different, increase or decrease according to this ratio).