Mouse BAFFR / TNFRSF13C ELISA Kit

Catalog No.: BEK1342

Size: 96T

Range: 78 pg/ml - 5000 pg/ml

Sensitivity < 20 pg/ml

Storage and Expiration: Store at 2-8℃ for 6 months, or at -20℃ for 12 months.

Application: For quantitative detection of BAFFR / TNFRSF13C in mouse serum, plasma, body fluids, tissue lysates or cell culture supernatants.

Introduction

BAFF receptor (B-cell activating factor receptor, BAFF-R), also known as tumor necrosis factor receptor superfamily member 13C (TNFRSF13C) and BLyS receptor 3 (BR3), is a membrane protein of the TNF receptor superfamily which recognizes BAFF, an essential factor for B cell maturation and survival. In humans it is encoded by the TNFRSF13C gene. B-cell activating factor (BAFF) enhances B-cell survival in vitro and is a regulator of the peripheral B-cell population. The protein encoded by this gene is a receptor for BAFF and is a type III transmembrane protein containing a single extracellular phenylalanine-rich domain. It is thought that this receptor is the principal receptor required for BAFF-mediated mature B-cell survival. 

Principle of the Assay

This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti- BAFFR / TNFRSF13C polyclonal antibody was pre-coated onto 96-well plates. And the biotin conjugated anti- BAFFR / TNFRSF13C polyclonal antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the BAFFR / TNFRSF13C amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of BAFFR / TNFRSF13C can be calculated.

Kit components

1. One 96-well plate pre-coated with anti-mouse BAFFR / TNFRSF13C antibody
2. Lyophilized mouse BAFFR / TNFRSF13C standards: 2 tubes (10 ng / tube)                     
3. Sample / Standard diluent buffer: 30ml
4. Biotin conjugated anti-mouse BAFFR / TNFRSF13C antibody (Concentrated): 130μl. Dilution: 1:100
5. Antibody diluent buffer: 12ml
6. Avidin-Biotin-Peroxidase Complex (ABC) (Concentrated): 130μl. Dilution: 1:100
7. ABC diluent buffer: 12ml
8. TMB substrate: 10ml
9. Stop solution: 10ml
10. Wash buffer (25X): 30ml
11. Note: Reconstitute standards and test samples with Kit Component 3.

Material Required But Not Provided

1. 37℃ incubator
2. Microplate reader (wavelength: 450nm)
3. Precise pipette and disposable pipette tips
4. Automated plate washer
5. ELISA shaker
6. 1.5ml of Eppendorf tubes
7. Plate cover
8. Absorbent filter papers
9. Plastic or glass container with volume of above 1L