2×SYBR Green qPCR Mix
Catalog# BPC1149-1
Lot # Check on the product label
Size: 50 Tx 50 μl
Storage and Expiration: Store at -20°C for 6 months.
Description:
The components of 2 x SYBR Green qPCR Mix include Hot-start enzyme named HotMaster Taq DNA Polymerase, dNTP, PCR reaction buffer, BSA and SYBR Green , and so on. The fluorescent dye SYBR Green I in the master mix enables the analysis of many different targets without having to synthesize target specific labeled probes. High specificity and sensitivity in PCR are achieved by the use of the hot-start enzyme HotMaster Taq DNA Polymerase together with a specialized PCR buffer.
Kit Components
|
Component |
Size |
|
2 x SYBR qPCR Mix |
1.25 ml |
Recommended PCR conditions (Example: 25&50 µl, prepare the reaction solution on ice)
|
Components |
Volume （25 μl） |
Volume （50 μl） |
Final Concentration |
|
2 x SYBR qPCR Mix |
12.5 μl |
25 μl |
1× |
|
DNA Template |
1 μl |
2 μl |
as required |
|
Forward Primer (10 μM) |
0.5 μl |
1 μl |
0.2 μM each |
|
Reverse Primer (10 μM) |
0.5 μl |
1 μl |
0.2 μM each |
|
ddH 2 O to final volume |
25 μl |
50 μl |
Not applicable |
