Zinc Assay Kit (Complexation colorimetry)

Catalog# BAR1013
Size: R1: 40 ml*1; R2: 10 ml*1
Lot # Check on the product label
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Price: $150.00

  


Catalog# BAR1013


Size: R1: 40 ml*1; R2: 10 ml*1


Lot # Check on the product label


Introduction

Use for the vitro quantitative determination of Zn ions in serum, plasma, or urine.


Principle

Zn ions in samples reacted with 5-Br-PAPS in reagent at RT, forming colored complex. The degree of color is in proportion to the concentration of Zn ions in the range of 400µg/dl.


Kit Components

 

Components

Final Concentration

Storage

 

Reagent A

(R1)

Ascorbic acid

50mmol/L

 

 

Store at 2-8 in dark for one year.

4-(2-ethoxyl)-1-piperazine-ethanesulfonic acid(Hepes)buffer(pH6.0)

200mmol/L

 Sodium Citrate

0.2mol/L

 

Reagent B

(R2)

2-(5-Bromo-2-pyridylazo)-5-[N-n-propyl-N-(3-sulfopropyl)amino]phenol, disodium salt, dihydrate(short for 5-Br-PAPS)

20µmol/L

Details