Monoclonal Anti- Neuron-specific class III β-tubulin, β3-tubulin Antibody

Catalog# BMA1045  

Lot # Check on the product label

Size 100 μg

Isotype IgG2a

Clone # T28

Host Mouse

Reactivity

Human, mouse, rat

Product Form Liquid

Purification Peptide affinity purified

Immunogen 

A synthetic peptide (conjugated with KLH) corresponding to the N-terminal of β3-tubulin.

Recommend Application

Western Blot, WB (1:500-1:2,000)

Immunohistochemistry, IHC-P(1:50-1:100)

Immunocytochemistry, ICC (1:200-1:500)

Flow Cytometry, Flow-Cyt (1:20-1:50)

Other applications have not been tested.

The optimal dilutions should be determined by end user.

Storage Buffer

1*PBS (pH7.4), 0.2% BSA, 40% Glycerol and 0.05% Sodium Azide.

Storage Instruction 

Store at 4°C after thawing (1 week). Aliquot and store at -20°C for long term (at least one year).

Avoid repeated freeze and thaw cycles.

Background

Tubulin is one of several members of a small family of globular proteins. The most common members of the tubulin family are α-tubulin and β-tubulin, each has a molecular weight of approximately 55 kDa. Neuron-specific class Ⅲ β-Tubulin, also called TUBB3, is a protein that in humans is encoded by the TUBB3 gene which maps to chromosome 16q24.3. It was significantly expressed in fibroblasts. And it has an important function in antimicrotubule drug resistance. 

Reference

1. Venter JC (Jun 1993). "Identification of new human receptor and transporter genes by high throughput cDNA (EST) sequencing". J Pharm Pharmacol. 45 Suppl 1: 355–60.

2. Ranganathan S, Dexter DW, Benetatos CA, Hudes GR (Mar 1998). "Cloning and sequencing of human betaIII-tubulin cDNA: induction of betaIII isotype in human prostate carcinoma cells by acute exposure to antimicrotubule agents". Biochim Biophys Acta 1395 (2): 237–45.