Monoclonal Anti- Tri-Methyl-Histone H3 / Lys9 Antibody

Catalog# BMA1043  

Lot # Check on the product label

Size 100 μg

Isotype IgG1

Clone # S49-8

Host Mouse

Reactivity Human, mouse, rat

Product Form Liquid

Purification Protein G purified

Immunogen 

A synthetic peptide (conjugated with KLH) corresponding to the region of Lys9.

Recommend Application

Western Blot, WB (1:2,000-1:5,000)

Immunohistochemistry, IHC-P(1:100)

Immunocytochemistry, ICC (1:200-1:500)

Other applications have not been tested.

The optimal dilutions should be determined by end user.

Storage Buffer

1*PBS (pH7.4), 0.2% BSA, 40% Glycerol and 0.05% Sodium Azide.

Storage Instruction 

Store at 4°C after thawing (1 week). Aliquot and store at -20°C for long term (at least one year).

Avoid repeated freeze and thaw cycles.

Background

Histones are the basic nuclear proteins responsible for the nucleosome structure within the chromosomal fiber in eukaryotes. Five classes of histone genes have been reported , Histone H3 is one of them. Histone proteins are highly post-translationally modified however Histone H3 is the most extensively modified of the five histones. It is an important protein in the emerging field of epigenetics, where its sequence variants and variable modification states are thought to play a role in the dynamic and long term regulation of genes.

Reference

1. Bhasin M, Reinherz EL, Reche PA (2006). "Recognition and classification of histones using support vector machine". J. Comput. Biol. 13 (1): 102–12.

2. Fischle, W., Tseng, B. S., Dormann, H. L., Ueberheide, B. M., Garcia, B. A., Shabanowitz, J., Hunt, D. F., Funabiki, H., Allis, C. D. Regulation of HP1-chromatin binding by histone H3 methylation and phosphorylation. Nature 438: 1116-1122, 2005.