Monoclonal Anti- B-cell lymphoma 2, Bcl-2 Antibody
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Catalog# |
BMA1007 |
|---|---|
|
Lot # |
Check on the product label |
|
Size |
100 μg |
| Isotype | IgG2b |
|
Clone # |
D24-5 |
| Host |
Mouse |
|
Reactivity |
Human, mouse |
| Product Form |
Liquid |
|
Purification |
Protein A purified |
|
Storage Buffer |
1*PBS (pH7.4), 0.2% BSA, 40% Glycerol and 0.05% Sodium Azide. |
| Immunogen | Recombinant protein within human BCL-2. |
|
Recommend Application |
Western Blot,WB (1:50) Immunohistochemistry, IHC-P (1:100) Immunocytochemistry, ICC (1:100-200) Other applications have not been tested. The optimal dilutions should be determined by end user. |
|
Storage Instruction |
Store at 4°C after thawing (1 week). Aliquot and store at -20°C for long term (at least one year). Avoid repeated freeze and thaw cycles. |
|
Background |
Bcl-2 (B-cell lymphoma 2) is the founding member of the Bcl-2 family of apoptosis regulator proteins encoded by the BCL2 gene. Bcl-2 is an important regulator of photoreceptor cell death in retinal degenerations. The bcl-2 protooncogene, which protects various cell types from apoptotic cell death, is expressed in the developing and adult nervous system. Bcl-2 may play an important role in survival of neurons both during development and throughout adult life. |
|
Reference |
1. Tsujimoto Y, Finger LR, Yunis J, Nowell PC, Croce CM (November 1984). "Cloning of the chromosome breakpoint of neoplastic B cells with the t(14;18) chromosome translocation". Science 226 (4678): 1097–99. 2. Cleary ML, Smith SD, Sklar J (October 1986). "Cloning and structural analysis of cDNAs for bcl-2 and a hybrid bcl-2/immunoglobulin transcript resulting from the t(14;18) translocation". Cell 47 (1): 19–28. 3. Chen, J., Flannery, J. G., LaVail, M. M., Steinberg, R. H., Xu, J., Simon, M. I. Bcl-2 overexpression reduces apoptotic photoreceptor cell death in three different retinal degenerations. Proc. Nat. Acad. Sci. 93: 7042-7047, 1996. 4. Farlie, P. G., Dringen, R., Rees, S. M., Kannourakis, G., Bernard, O. bcl-2 transgene expression can protect neurons against developmental and induced cell death. Proc. Nat. Acad. Sci. 92: 4397-4401, 1995. |
