|
Catalog# |
BRP1196-1 |
|---|---|
|
Lot # |
Check on the product label |
|
Size |
10 μg |
|
Description |
Recombinant mouse IL-1RA is produced by the E.coli system, with the target gene encoding Arg27-Gln178 expressed. |
|
Synonyms |
IL1RN, DIRA, ICIL-1RA, IL-1RN, IL-1ra, IL-1ra3, IL1F3, IL1RA, IRAP, MVCD4, interleukin 1 receptor antagonist |
|
Source |
E.coli |
|
Uniprot No. |
P25085 |
|
Purity |
>95% by SDS-PAGE |
|
Endotoxin level |
<1 EU/µg by LAL test |
|
Formulation |
Lyophilized from a 0.2 μm filtered solution of 20mM Histidine-HCl, 10% Trehalose, 0.05% Tween 80, pH5.3. |
|
Reconstitution |
Dissolve the lyophilized protein in distilled water (It is not recommended to reconstitute to a concentration <100 μg/ml.) |
|
Application |
SDS-PAGE Other applications have not been tested. The optimal dilutions should be determined by end user. |
|
Storage& Expiration |
Ship at 4℃. Upon receipt, aliquot and store at -20℃ or -80℃ for long term. Avoid repeated freeze and thaw cycles. |
|
Background |
The interleukin-1 receptor antagonist (IL-1RA) is a protein that in humans is encoded by the IL1RN gene. IL-1RA was initially called the IL-1 inhibitor and was discovered separately in 1984 by two independent laboratories. IL-1RA is an agent that binds non-productively to the cell surface interleukin-1 receptor (IL-1R), the same receptor that binds interleukin 1 family (IL-1), preventing IL-1's from sending a signal to that cell. |
|
Reference |
1. Steinkasserer A, Spurr NK, Cox S, Jeggo P, Sim RB (July 1992). "The human IL-1 receptor antagonist gene (IL1RN) maps to chromosome 2q14-q21, in the region of the IL-1 alpha and IL-1 beta loci". Genomics. 13 (3): 654–657. 2. Patterson D, Jones C, Hart I, Bleskan J, Berger R, Geyer D, et al. (January 1993). "The human interleukin-1 receptor antagonist (IL1RN) gene is located in the chromosome 2q14 region". Genomics. 15 (1): 173–176. 3. Dinarello CA (December 1994). "The interleukin-1 family: 10 years of discovery". FASEB Journal. 8 (15): 1314–1325. |
