Recombinant Human IL-17A Protein

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Catalog#

BRP1188-1

Lot #

Check on the product label

Size

10 μg

Description

Recombinant human IL-17A is produced by the mammalian system, with the target gene encoding Gly24-Ala155 expressed with 6x His-tag at the C-terminus.

Synonyms

IL17A, CTLA8, IL-17, IL-17A, IL17, CTLA-8, interleukin 17A, ILA17, Interleukin 17A

Source

Human Cells

Uniprot No.

Q16552

Purity

>95% by SDS-PAGE

Endotoxin level

<0.01 EU/µg by LAL test

Formulation

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

Reconstitution

Dissolve the lyophilized protein in distilled water (It is not recommended to reconstitute to a concentration <100 μg/ml.)

Application

SDS-PAGE

Other applications have not been tested.

The optimal dilutions should be determined by end user.

Storage& Expiration

Ship at 4℃. Upon receipt, aliquot and store at -20℃ or -80℃ for long term.

Avoid repeated freeze and thaw cycles.

Background

Interleukin-17A is a protein that in humans is encoded by the IL17A gene. In rodents, IL-17A used to be referred to as CTLA8, after the similarity with a viral gene (O40633). The protein encoded by this gene is a proinflammatory cytokine produced by activated T cells. This cytokine regulates the activities of NF-kappaB and mitogen-activated protein kinases. This cytokine can stimulate the expression of IL6 and cyclooxygenase-2 (PTGS2/COX-2), as well as enhance the production of nitric oxide (NO).

Reference

1. Rouvier E, Luciani MF, Mattéi MG, Denizot F, Golstein P (June 1993). "CTLA-8, cloned from an activated T cell, bearing AU-rich messenger RNA instability sequences, and homologous to a herpesvirus saimiri gene". Journal of Immunology. 150 (12): 5445–5456.

2. Yao Z, Painter SL, Fanslow WC, Ulrich D, Macduff BM, Spriggs MK, Armitage RJ (December 1995). "Human IL-17: a novel cytokine derived from T cells". Journal of Immunology. 155 (12): 5483–5486.

Details