Cytokine Kits
Cytokine Kits
Cytokines are a group of low molecular weight (approximately 6-70kDa) soluble proteins secreted by various cells such as lymphocytes, macrophages, natural killer (NK) cells, mast cells, and stromal cells. Different cell types can secrete the same cytokine, and a single cytokine can act on various cell types. Cytokines play a crucial role in immune responses and are important mediators of the immune system's communication network. Cytokines are involved in the pathophysiological processes of viral and bacterial infections, as well as various diseases, including cancer, autoimmune diseases, neurodegenerative diseases, and cytokine storms caused by cell therapies, which have gained significant attention recently. As a result, they have become a focus of medical research.
Product Advantages
Homogeneous, no-wash reaction model,convenient and efficient, compatible with automated instruments.
Wide dynamic range,small sample volume required, suitable for high-throughput rapid testing.
High sensitivity and strong anti-interference ability, stable performance and reliable reproducibility.
High sample compatibility,applicable to cell culture supernatants, serum, and other natural samples.
Application
Biospes has launched several cytokine kits based on HiLA methods. These kits can quantitatively detect cytokine levels in cell culture supernatants or serum and serve as an efficient alternative to traditional methods such as ELISA. They are applicable in various research areas, including basic research, drug immunogenicity evaluation, vaccine development, and more.
Detection Principle of Assay
HiLA is a homogeneous immunoassay method based on energy transfer between acceptor and donor beads. This series of kits uses a dual antibody sandwich homogeneous chemiluminescence method to determine cytokine concentrations. Taking the Human IL-6 (hIL-6) kit as an example: Incubate the acceptor beads coated with anti- IL-6 antibody 1, biotin conjugated anti- IL-6 antibody 2, and the sample to form a sandwich immune complex (antibody 1-sample-antibody 2). The immune complex is then incubated with donor beads coated with streptavidin to form a chemiluminescent complex, ensuring that the distance between the two beads is less than 200 nm. When the distance between the two beads is less than 200 nm, the donor beads is excited by light (680 nm), producing singlet oxygen which diffuses to the acceptor beads, inducing energy transfer. The acceptor beads absorb this energy and emit scattering light at 615 nm. The light signal is collected by a photosensitive detector, and the concentration of IL-6 in the sample is calculated through mathematical fitting. In the absence of human IL-6 in the sample, no immune complex is formed, or the distance between the two beads exceeds 200 nm, exceeding the transfer distance for singlet oxygen, and the acceptor beads do not emit any light.
Protocol
In the protocol, 10 μL of sample and pre-mixed R1 and R2 kits are added and incubated at 37°C for 15 minutes. After adding R3 kit in the dark, the mixture is incubated for an additional 10 minutes before detection. Compared to traditional ELISA kits, this detection process eliminates the need for repeated washing of plates, simplifies operations, and significantly reduces labor and time costs.
Cytokine Kits
