Recombinant SARS-CoV-2 Nucleocapsid Protein (Omicron-BA.1)

Catalog# BRP1149

Lot # Check on the product label

Size 1 mg

Description

Recombinant SARS-CoV-2 Nucleocapsid Protein (Omicron-BA.1) with his tag.

Source E.coli

Purity >95% by SDS-PAGE

Molecular Weight 46.7kDa

Formulation Liquid

Application

Western Blot

ELISA

Other applications have not been tested.

The optimal dilutions should be determined by end user.

Storage buffer

0.01M PBS, 0.3M NaCl pH7.0, 0.1% Proclin300.

Storage & Expiration

Ship at 4℃. Upon receipt, aliquot and store at -20℃ for long term.

Avoid repeated freeze and thaw cycles.

Background

Omicron (BA.1) has more mutations than any previous SARS-CoV-2 variant with 50 mutations and approximately 32 of these pertain to the spike protein which most vaccines target to neutralise the virus. Many of the mutations are novel and are not found in previous variants of the virus. The variant is characterised by 30 amino acid changes, three small deletions, and one small insertion in the spike protein compared with the original virus, of which 15 are located in the receptor-binding domain (residues 319–541). It also carries a number of changes and deletions in other genomic regions. Additionally, the variant has three mutations at the furin cleavage site which facilitate transmission of the virus.

Reference

1. Khandia R, Singhal S, Alqahtani T, Kamal MA, El-Shall NA, Nainu F, et al. (June 2022). "Emergence of SARS-CoV-2 Omicron (B.1.1.529) variant, salient features, high global health concerns and strategies to counter it amid ongoing COVID-19 pandemic". Environmental Research. 209: 112816.

2. Poudel S, Ishak A, Perez-Fernandez J, Garcia E, León-Figueroa DA, Romaní L, et al. (2022). "Highly mutated SARS-CoV-2 Omicron variant sparks significant concern among global experts - What is known so far". Travel Medicine and Infectious Disease. 45: 102234.

3. Khandia R, Singhal S, Alqahtani T, Kamal MA, El-Shall NA, Nainu F, et al. (June 2022). "Emergence of SARS-CoV-2 Omicron (B.1.1.529) variant, salient features, high global health concerns and strategies to counter it amid ongoing COVID-19 pandemic". Environmental Research. 209: 112816.

4. Callaway E (December 2021). "Heavily mutated Omicron variant puts scientists on alert". Nature. 600 (7887): 21. Bibcode:2021Natur.600.21C.