7-AAD Viability Staining Buffer

Catalog # BAD1010

Size: 100T

Lot # Check on the product label

Description

7-Aminoactinomycin D (7-AAD) is a fluorescent chemical compound with a strong affinity for DNA. It is used as a fluorescent marker for DNA in fluorescence microscopy and flow cytometry. It intercalates in double-stranded DNA, with a high affinity for GC-rich regions, making it useful for chromosome banding studies. With an absorption maximum at 546 nm, 7-AAD is efficiently excited using a 543 nm helium-neon laser; it can also be excited with somewhat lower efficiency using a 488 nm or 514 nm argon laser lines.

Storage Buffer

In PBS (pH7.2), 0.09% NaN₃

Storage

Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.

Recommended Application

Flow cytometry      

Usage

This reagent is used to analyze cell cycle by flow cytometry. It need not dilution. Use 5 μL /test and incubate for 5 minutes at room temperature before analysis.