Custom polyclonal antibodies
For polyclonal antibodies, we can manufacture rabbit polyclonal, mouse polyclonal & goat polyclonal antibodies. In addition, the immunogen can be synthesized peptide or recombinant protein, customers can choose according to their own assays and requirements, Please check the following details:
Necessary Info1. Full name of the gene, NCBI # or Uniprot ID.
2. If possible, provide us the full sequence of the gene (optional).
3. Expected application: ELISA? WB? IHC? ICC? IF? Flow Cyto?.....
4. What sample the antibody plan to detect? Please indicated this in detail, e.g. A431 cell lysate, mouse lung tissue, etc.
5. Other requirements: like need tag at the sequence; just need anti-serum? Manufacture according to the customers' sequence, etc.
Protocol1. Analyze the sequence, design and sythesize the peptides (2 different peptides or more to ensure the success, 15-20aa, if need longer, please indicate, price will be increased for more than 20aa).2. Conjugated the peptides with the carrier, usually the KLH or BSA.3. Immunize rabbits (usually at least 4 rabbits), mouse, or goat with the peptides (Multiple immunization for each rabbit, mouse or goat), during this period, we need collect the sample and test to determined the immunization.4. Collect the anti-serum after immunization and test.5. Purify the antibody from the anti-serum if the results are good (if the testing results are not good, we will immunize again).
Quality Control1. ELISA (1:20000-100000) with high stability & sensitivity
2. The expected application with the same or the similar samples of customers
Time75 days (maybe prolonged or decreased for different antibodies)
Provided products1. Purified antibody, 10-50mg. (For goat polyclonal, will be greater than 100mg)2. All the rest anti-serum (End user can use this for testing or purifying the antibody) or the ascites for mouse polyclonal, 10ml. (For goat polyclonal, will be greater than 100ml)3. Rest peptide / immunogen, 3mg.4. Report for the whole service & manual
Price1. Rabbit polyclonal: Please inquiry2. Mouse polyclonal: Please inquiry3. Goat polyclonal: Please inquiry
Others1. If customer can provide the peptide (requirements: volume: 3-5mg for each gene, purity > 85%), price will be cheaper.2. If customer just need anti-serum, not the purified antibodies, the price will be different, please inquiry and indicate at the beginning.3. We respect the intellectual property of the antibody, will not sell it commercially unless get the permit from customer.
Please note: The above information is for custom polyclonal antibodies which used the synthesized peptide as the immunogen, we also provide the custom polyclonal antibodies service which used the recombinant protein as immunogen, please inquiry for the details.